The mechanisms of vesicle budding and fusion

Cell. 2004 Jan 23;116(2):153-66. doi: 10.1016/s0092-8674(03)01079-1.


Genetic and biochemical analyses of the secretory pathway have produced a detailed picture of the molecular mechanisms involved in selective cargo transport between organelles. This transport occurs by means of vesicular intermediates that bud from a donor compartment and fuse with an acceptor compartment. Vesicle budding and cargo selection are mediated by protein coats, while vesicle targeting and fusion depend on a machinery that includes the SNARE proteins. Precise regulation of these two aspects of vesicular transport ensures efficient cargo transfer while preserving organelle identity.

Publication types

  • Review
  • Comment

MeSH terms

  • Animals
  • Biological Transport
  • Endoplasmic Reticulum / metabolism
  • Golgi Apparatus / metabolism
  • Humans
  • Membrane Fusion
  • Membrane Proteins / physiology*
  • Models, Biological
  • Models, Molecular
  • Organelles / physiology
  • SNARE Proteins
  • Saccharomyces cerevisiae / physiology
  • Saccharomyces cerevisiae / ultrastructure
  • Temperature
  • Vesicular Transport Proteins*


  • Membrane Proteins
  • SNARE Proteins
  • Vesicular Transport Proteins