Expression of connective tissue growth factor after glaucoma filtration surgery in a rabbit model

Invest Ophthalmol Vis Sci. 2004 Feb;45(2):485-91. doi: 10.1167/iovs.03-0485.


Purpose: Connective tissue growth factor (CTGF) appears to play a significant role in mediating fibrosis in several tissues. To gain further understanding of the role of CTGF in the scar formation that occurs after glaucoma filtering surgery (GFS), experiments were performed in a rabbit model.

Methods: . Three experiments were performed: (1) CTGF and transforming growth factor (TGF)-beta expression were measured quantitatively after GFS, using ELISA. (2) After GFS conjunctival bleb tissues were immunostained for the presence of CTGF and TGF-beta. (3) Exogenous CTGF was injected into mitomycin-C (MMC)-treated filtering blebs and the scaring response compared to TGF-beta and physiological saline-injected blebs.

Results: CTGF and TGF-beta were expressed maximally by day 5 after surgery and were both shown to be present in the bleb tissues after GFS. The addition of exogenous CTGF and TGF-beta increased the rate of failure of GFS blebs.

Conclusions: These data support the hypothesis that CTGF plays an important role in scarring and wound contracture after GFS. Inhibition of CTGF synthesis or its action may help prevent bleb failure and improve long-term GFS outcomes.

Publication types

  • Research Support, Non-U.S. Gov't
  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • Animals
  • Conjunctiva / drug effects
  • Conjunctiva / metabolism*
  • Conjunctiva / pathology
  • Connective Tissue Growth Factor
  • Enzyme-Linked Immunosorbent Assay
  • Filtering Surgery*
  • Glaucoma / metabolism*
  • Glaucoma / surgery
  • Immediate-Early Proteins / metabolism*
  • Immediate-Early Proteins / pharmacology
  • Immunohistochemistry
  • Intercellular Signaling Peptides and Proteins / metabolism*
  • Intercellular Signaling Peptides and Proteins / pharmacology
  • Male
  • Models, Animal
  • Rabbits
  • Trabecular Meshwork / drug effects
  • Trabecular Meshwork / metabolism*
  • Trabecular Meshwork / pathology
  • Transforming Growth Factor beta / metabolism
  • Transforming Growth Factor beta / pharmacology


  • Immediate-Early Proteins
  • Intercellular Signaling Peptides and Proteins
  • Transforming Growth Factor beta
  • Connective Tissue Growth Factor