Identification of a novel left-right asymmetrically expressed gene in the mouse belonging to the BPI/PLUNC superfamily

Dev Dyn. 2004 Feb;229(2):373-9. doi: 10.1002/dvdy.10450.


In the process of left-right (L-R) axis formation in the mouse, the node plays a critical role as a structure where the initial breaking of L-R symmetry occurs. Here, we report on the gene LPlunc1, a member of BPI/PLUNC gene superfamily, which is asymmetrically expressed in the developing mouse node. LPlunc1 protein is secreted as a processed form of relative molecular mass 54K-60K and shares sequence features with the other members of BPI/PLUNC superfamily, including the N-terminal and C-terminal homology domains, each of which is considered to form a lipid binding pocket. LPlunc1 is transiently expressed in the crown cells of the node asymmetrically. This expression pattern of Lplunc1 highly overlaps with that of Nodal, a major player during the L-R formation. Interestingly, this asymmetric expression pattern is randomized in the iv mutant and reversed in the inv mutant, indicating that LPlunc1 is downstream of iv and inv. Our results suggest a link between lipid binding/transfer and the axis development.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Embryo, Mammalian / cytology
  • Embryo, Mammalian / metabolism*
  • Gene Expression Regulation, Developmental / physiology*
  • Glycoproteins / genetics*
  • Glycoproteins / metabolism
  • In Situ Hybridization
  • Mice
  • Mice, Mutant Strains
  • Nodal Protein
  • Organ Specificity
  • Phosphoproteins / genetics*
  • Phosphoproteins / metabolism
  • Transforming Growth Factor beta / genetics*
  • Transforming Growth Factor beta / metabolism


  • Bpifa1 protein, mouse
  • Glycoproteins
  • Nodal Protein
  • Nodal protein, mouse
  • Phosphoproteins
  • Transforming Growth Factor beta