Presence of Multiple Human Papillomavirus Types in Cervical Samples From HIV-infected Women

Gynecol Oncol. 2004 Jan;92(1):225-31. doi: 10.1016/j.ygyno.2003.10.004.

Abstract

Objectives: The aim of this study was to detect and identify human papillomavirus (HPV) genotypes on a population of women infected by the human immunodeficiency virus (HIV) and to investigate the role of multiple infections on cervical dysplasia.

Methods: Two hundred and fifty-five HIV-infected women were enrolled on a study to evaluate the prevalence of HPV and cervical intra-epithelial neoplasia (CIN). A group of HIV-negative women with confirmed CIN diagnosis was included for comparison. A polymerase chain reaction (PCR)-reverse hybridization method was applied to detect and precisely identify HPV types, specifically multiple infections.

Results: On HIV patients, an altered Pap smear confirmed by biopsy was observed on 45 (18%); HPV-DNA prevalence was 87% (223/255), with 45% (116/255) infected by more than two types. In contrast, HPV-DNA was detected in all 36 women of the control group but only 3 were infected by more than two types. Cervical dysplasia was associated with low CD4 counts and elevated high-risk HPV viral load. However, the presence of multiple HPV types did not correlate with the degree of immune suppression or the presence of cervical lesions.

Conclusions: Infection with multiple HPV types is a rather frequent finding on Brazilian HIV-infected women. On this population, concomitant infection with three or more HPV types does not seem to confer an additional risk of cervical dysplasia in comparison to single/double infections, nor to be related to more severe immunesuppresion.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Adolescent
  • Adult
  • Aged
  • Cervical Intraepithelial Neoplasia / immunology
  • Cervical Intraepithelial Neoplasia / virology*
  • Female
  • Genotype
  • HIV Infections / complications*
  • HIV Infections / immunology
  • HIV Infections / virology
  • Humans
  • Middle Aged
  • Papillomaviridae / genetics*
  • Papillomavirus Infections / complications*
  • Papillomavirus Infections / immunology
  • Papillomavirus Infections / virology
  • Polymerase Chain Reaction
  • Tumor Virus Infections / complications*
  • Tumor Virus Infections / immunology
  • Tumor Virus Infections / virology
  • Uterine Cervical Neoplasms / immunology
  • Uterine Cervical Neoplasms / virology*
  • Viral Load