Novel cell line selectively expressing neuropeptide Y-Y2 receptors

J Recept Signal Transduct Res. 2003;23(4):351-60. doi: 10.1081/rrs-120026974.

Abstract

Neuropeptide Y (NPY) recognition by the human neuroblastoma cell lines SiMa, Kelly, SH-SY5Y, CHP-234, and MHH-NB-11 was analyzed in radioactive binding assays using tritiated NPY. For the cell lines CHP-234 and MHH-NB-11 binding of [3H]propionyl-NPY was observed with Kd-values of 0.64 +/- 0.07 nM and 0.53 +/- 0.12 nM, respectively, determined by saturation analysis with non-linear regression. The receptor subtype was determined by competition analysis using the subtype selective NPY analogues [Leu31, Pro34]-NPY (NPY-Y1, NPY-Y5), [Ahx(5-24)]-NPY (NPY-Y2), [Ala31, Aib32]-NPY (NPY-Y5), NPY [3-36] (NPY-Y2, NPY-Y5), and NPY [13-36] (NPY-Y2). Both cell lines, CHP-234 and MHH-NB-11, the latter one being characterized for NPY receptors for the first time, showed exclusive expression of NPY-Y2 receptors. In both cell lines binding of NPY induced signal transduction, which was monitored as reduction of forskolin-induced cAMP production in an ELISA.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Amino Acid Sequence
  • Binding, Competitive
  • Cell Line, Tumor
  • Cyclic AMP / metabolism
  • Dose-Response Relationship, Drug
  • Enzyme Inhibitors / pharmacology
  • Enzyme-Linked Immunosorbent Assay
  • Humans
  • Kinetics
  • Linear Models
  • Molecular Sequence Data
  • Peptide Biosynthesis
  • Peptides / chemistry
  • Protein Binding
  • Receptors, Neuropeptide Y / biosynthesis*
  • Sequence Homology, Amino Acid
  • Signal Transduction

Substances

  • Enzyme Inhibitors
  • Peptides
  • Receptors, Neuropeptide Y
  • neuropeptide Y2 receptor
  • Cyclic AMP