Factorial screening of antibody purification processes using three chromatography steps without protein A

J Chromatogr A. 2004 Jan 23;1024(1-2):79-85. doi: 10.1016/j.chroma.2003.10.060.


Protein A affinity chromatography is often employed as a capture step to meet the purity, yield, and throughput requirements for pharmaceutical antibody purification. However, a trade-off exists between step performance and price. Protein A resin removes 99.9% of feed stream impurities; however, its price is significantly greater than those of non-affinity media. With many therapeutic indications for antibodies requiring high doses and/or chronic administration, the consideration of process economics is critical. We have systematically evaluated the purification performance of cation-exchange, anion-exchange, hydroxyapatite, hydrophobic interaction, hydrophobic charge induction, and small-molecule ligand resins in each step of a three-step chromatographic purification process for a CHO-derived monoclonal antibody. Host cell proteins were removed to less-than-detectable for three processes (cation-exchange-anion-exchange-hydrophobic interaction chromatography, cation-exchange-anion-exchange-mixed cation-exchange chromatography, and cation-exchange-mixed cation-exchange-anion-exchange chromatography). The order of the process steps affected purification performance significantly.

MeSH terms

  • Animals
  • Antibodies, Monoclonal / chemistry
  • Antibodies, Monoclonal / isolation & purification*
  • CHO Cells
  • Chromatography, Affinity / methods*
  • Chromatography, Ion Exchange / methods*
  • Cricetinae
  • Enzyme-Linked Immunosorbent Assay
  • Humans
  • Recombinant Proteins / chemistry
  • Recombinant Proteins / isolation & purification
  • Staphylococcal Protein A / chemistry*


  • Antibodies, Monoclonal
  • Recombinant Proteins
  • Staphylococcal Protein A