Identification of MAPK substrates by expression screening with solid-phase phosphorylation

Methods Mol Biol. 2004;250:211-36. doi: 10.1385/1-59259-671-1:211.

Authors

Rikiro Fukunaga¹, Tony Hunter

Affiliation

¹ Department of Genetics, Osaka University Medical School, Osaka, Japan.

PMID: 14755091
DOI: 10.1385/1-59259-671-1:211

No abstract available

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Animals
Autoradiography
Bacteriophage lambda / genetics
DNA, Complementary / genetics
Genetic Vectors
HeLa Cells
Humans
In Vitro Techniques
MAP Kinase Signaling System
Mitogen-Activated Protein Kinase 3
Mitogen-Activated Protein Kinases / genetics
Mitogen-Activated Protein Kinases / isolation & purification
Mitogen-Activated Protein Kinases / metabolism*
Peptide Library*
Phosphorylation
Plasmids / genetics
Recombinant Proteins / genetics
Recombinant Proteins / isolation & purification
Recombinant Proteins / metabolism
Rosaniline Dyes
Staining and Labeling
Substrate Specificity

Substances

DNA, Complementary
Peptide Library
Recombinant Proteins
Rosaniline Dyes
Coomassie blue
Mitogen-Activated Protein Kinase 3
Mitogen-Activated Protein Kinases