1,25-Dihydroxyvitamin D3 inhibits thromboxane release from activated macrophages

Res Commun Chem Pathol Pharmacol. 1992 Nov;78(2):235-43.


1,25-Dihydroxyvitamin D3 (1,25-(OH)2D3) in concentrations from 0.1 to 10 nM suppressed immunoreactive thromboxane B2 (iTXB2) release from Propionibacterium acnes (P. acnes)-elicited liver adherent cells stimulated with lipopolysaccharide (LPS, 1 microgram/ml). These suppressive effects of 1,25-(OH)2D3 were also observed in oyster glycogen-elicited peritoneal macrophages. On the contrary, it did not inhibit iTXB2 release from both resident Kupffer cells and peritoneal macrophages stimulated with the same concentration of LPS. Furthermore, 1,24(R)-dihydroxyvitamin D3 (1,24(R)-(OH)2D3), a vitamin D3 analogue, also inhibited iTXB2 release from liver adherent cells, but, another synthesized vitamin D3 analogue, 1 alpha-hydroxyvitamin D3 (1 alpha-OH-D3) tended to decrease iTXB2 release only at higher concentrations. These results suggest that active vitamin D3 analogues inhibit iTXB2 release from activated macrophages.

Publication types

  • Comparative Study

MeSH terms

  • Animals
  • Calcitriol / pharmacology*
  • Dihydroxycholecalciferols / pharmacology
  • Endotoxins / administration & dosage
  • Kupffer Cells / drug effects
  • Lipopolysaccharides
  • Liver / drug effects
  • Macrophage Activation
  • Macrophages / metabolism*
  • Male
  • Mice
  • Mice, Inbred BALB C
  • Peritoneal Cavity
  • Propionibacterium acnes
  • Thromboxane B2 / antagonists & inhibitors*
  • Thromboxane B2 / metabolism


  • Dihydroxycholecalciferols
  • Endotoxins
  • Lipopolysaccharides
  • Thromboxane B2
  • 1 alpha,24-dihydroxyvitamin D3
  • Calcitriol