Effects of 6-gingerol, an antioxidant from ginger, on inducing apoptosis in human leukemic HL-60 cells

In Vivo. Nov-Dec 2003;17(6):641-5.


6-Gingerol, a naturally occurring plant phenol, is one of the major components of fresh ginger. In this paper, the antioxidative effects of 6-gingerol were detected by DPPH and DCFH assays and, as predicted, 6-gingerol as an antioxidant was shown to protect HL-60 cells from oxidative stress. Moreover, it induced cell death in promyelocytic leukemia HL-60 cells, caused DNA fragmentation and inhibited Bcl-2 expression in HL-60 cells. These results suggested that the inhibition of Bcl-2 expression in HL-60 cells might account for the mechanism of 6-gingerol-induced apoptosis. In the inhibitory assay, the cytotoxic effect of 6-gingerol could be prevented by catalase. We suggest that 6-gingerol induced cell death by mediating reactive oxygen species such as hydrogen peroxide and the superoxide anion. Therefore, the results showed that 6-gingerol induced apoptosis in HL-60 cells, not due to its antioxidative activity.

MeSH terms

  • Antioxidants / toxicity*
  • Apoptosis / drug effects*
  • Catechols
  • Cytotoxins / toxicity
  • Dose-Response Relationship, Drug
  • Fatty Alcohols / toxicity*
  • Flow Cytometry
  • Ginger*
  • HL-60 Cells
  • Humans
  • Reactive Oxygen Species / metabolism


  • Antioxidants
  • Catechols
  • Cytotoxins
  • Fatty Alcohols
  • Reactive Oxygen Species
  • gingerol