The histone-fold protein complex CHRAC-15/17 enhances nucleosome sliding and assembly mediated by ACF

Mol Cell. 2004 Jan 30;13(2):265-77. doi: 10.1016/s1097-2765(03)00523-9.


The histone fold is a structural motif with which two related proteins interact and is found in complexes involved in wrapping DNA, the nucleosome, and transcriptional regulation, as in NC2. We reveal a novel function for histone-fold proteins: facilitation of nucleosome remodeling. ACF1-ISWI complex (ATP-dependent chromatin assembly and remodeling factor [ACF]) associates with histone-fold proteins (CHRAC-15 and CHRAC-17 in the human chromatin accessibility complex [CHRAC]) whose functional relevance has been unclear. We show that these histone-fold proteins facilitate ATP-dependent nucleosome sliding by ACF. Direct interaction of the CHRAC-15/17 complex with the ACF1 subunit is essential for this process. CHRAC-17 interacts with another histone-fold protein, p12, in DNA polymerase epsilon, but CHRAC-15 is essential for interaction with ACF and enhancement of nucleosome sliding. Surprisingly, CHRAC-15/17, p12/CHRAC-17, and NC2 complexes facilitate ACF-mediated chromatin assembly by a mechanism different from nucleosome sliding enhancement, suggesting a general activity of H2A/H2B type histone-fold complexes in chromatin assembly.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Amino Acid Sequence
  • Animals
  • Chromatin / chemistry
  • DNA / chemistry
  • DNA Polymerase II / chemistry
  • DNA Polymerase III / chemistry
  • DNA-Binding Proteins / chemistry
  • Dose-Response Relationship, Drug
  • Drosophila
  • Glutathione Transferase / metabolism
  • Histones / chemistry*
  • Humans
  • Molecular Sequence Data
  • Nucleoproteins / chemistry
  • Nucleosomes / chemistry*
  • Protein Binding
  • Protein Folding
  • Protein Structure, Tertiary
  • Recombinant Fusion Proteins / metabolism
  • Sequence Homology, Amino Acid
  • Time Factors


  • CHRAC1 protein, human
  • Chromatin
  • DNA-Binding Proteins
  • Histones
  • Nucleoproteins
  • Nucleosomes
  • POLE3 protein, human
  • Recombinant Fusion Proteins
  • DNA
  • Glutathione Transferase
  • DNA Polymerase II
  • DNA Polymerase III