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, 42 (2), 627-30

Enzyme-linked Immunosorbent Assay for Helicobacter Pylori Needs Adjustment for the Population Investigated

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Enzyme-linked Immunosorbent Assay for Helicobacter Pylori Needs Adjustment for the Population Investigated

Thi Thu Ha Hoang et al. J Clin Microbiol.

Abstract

Helicobacter pylori infection and peptic ulcer disease are common in developing countries, e.g., Vietnam. An enzyme-linked immunosorbent assay (ELISA) for screening of patients and for seroepidemiology is a useful tool but needs to be validated in the population studied. We used in-house ELISA with sonicated Swedish and Vietnamese strains as antigens to measure immunoglobulin G antibodies after absorption with sonicated Campylobacter jejuni in sera from 270 H. pylori culture-confirmed peptic ulcer patients, 128 Swedish urea-breath test and immunoblot-positive healthy controls, and 432 Vietnamese immunoblot-positive population controls. Sonicated whole-cell antigen based on the local strains showed a significantly better performance. Immunoblot-positive peptic ulcer patients had significantly higher antibody concentrations than immunoblot-positive population controls, necessitating a lower cutoff level if serology is used for screening or epidemiological purposes. The study shows that the parameters of ELISA for H. pylori need to be adjusted for the population being investigated.

Figures

FIG. 1.
FIG. 1.
Reverse cumulative distribution curves showing optical density values of titers in sera from Vietnamese peptic ulcer patients and population controls (n = 701).
FIG. 2.
FIG. 2.
Receiver operating characteristics curves for sensitivity and specificity obtained with the antigens based on Vietnamese and Swedish strains. The evaluation was made on Vietnamese sera from the normal population (n = 431) and with immunoblot as the reference method.
FIG. 3.
FIG. 3.
Reverse cumulative distribution graph of the optical density values of antibody titers in ELISA with Vietnamese strains in immunoblot-positive Vietnamese peptic ulcer patients (n = 269) and population controls (n = 376).

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