Rapid identification of high affinity peptide ligands using positional scanning synthetic peptide combinatorial libraries

Biotechniques. 1992 Dec;13(6):901-5.


We describe here a conceptually unique set of individual synthetic peptide combinatorial libraries (SPCLs), termed a positional scanning SPCL (PS-SPCL), that can be used for the rapid (i.e., a single day) identification of peptide sequences that bind with high affinity to antibodies, receptors or other acceptor molecules. The PS-SPCL described here is made up of six individual positional peptide libraries, each one consisting of hexamers with a single position defined and five positions as mixtures. As an example of the utility of such PS-SPCLs, the antigenic determinants recognized by two different monoclonal antibodies were correctly identified upon a single screening.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Amino Acid Sequence
  • Antigens / genetics
  • Evaluation Studies as Topic
  • Gene Library*
  • Genetic Techniques
  • Ligands
  • Molecular Sequence Data
  • Peptides / chemical synthesis
  • Peptides / genetics*
  • Peptides / immunology


  • Antigens
  • Ligands
  • Peptides