PCR-SSCP: a method for detection of mutations

Genet Anal Tech Appl. 1992 Jun;9(3):73-9. doi: 10.1016/1050-3862(92)90001-l.

Abstract

PCR-SSCP (polymerase chain reaction-single-strand conformation polymorphism) analysis is one of the simplest and perhaps one of the most sensitive methods for detection of mutations based on PCR technology. The principles of PCR-SSCP, guidelines for experiments, and applications of this technique in various fields are reviewed.

Publication types

  • Comparative Study
  • Research Support, Non-U.S. Gov't
  • Review

MeSH terms

  • Bacteria / genetics
  • DNA Mutational Analysis / methods*
  • DNA, Bacterial / analysis
  • DNA, Bacterial / genetics
  • DNA, Single-Stranded / analysis
  • DNA, Single-Stranded / genetics
  • Electrophoresis, Polyacrylamide Gel / methods
  • False Negative Reactions
  • Genetic Diseases, Inborn / genetics
  • Genetic Markers
  • Humans
  • Neoplasms / genetics
  • Nucleic Acid Conformation
  • Polymerase Chain Reaction*
  • Polymorphism, Genetic*
  • Repetitive Sequences, Nucleic Acid
  • Sensitivity and Specificity

Substances

  • DNA, Bacterial
  • DNA, Single-Stranded
  • Genetic Markers