Red blood cell protein map: a comparison between carrier-ampholyte pH gradient and immobilized pH gradient, and identification of four red blood cell enzymes

Appl Theor Electrophor. 1992;3(2):77-82.


The aim of this study was (a) to establish a red blood cell (RBC) protein map with immobilized pH gradient for the first dimension (b) to compare the pattern with previously published RBC protein map obtained with carrier-ampholyte pH gradients and (c) to localize four new enzymes on the map (i.e. 6-phosphogluconic dehydrogenase, glyceraldehyde-3-phosphate dehydrogenase, glutathione peroxidase and superoxide dismutase). This publication provides the most updated RBC polypeptide pattern with twelve proteins or enzymes localized on the map.

Publication types

  • Comparative Study

MeSH terms

  • Blood Protein Electrophoresis / methods*
  • Blood Proteins / analysis*
  • Buffers*
  • Electrophoresis, Gel, Two-Dimensional / methods*
  • Erythrocytes / chemistry*
  • Erythrocytes / enzymology
  • Glucosephosphate Dehydrogenase / blood
  • Glutathione Peroxidase / blood
  • Humans
  • Hydrogen-Ion Concentration*
  • Isoelectric Focusing / methods*
  • Phosphogluconate Dehydrogenase / blood
  • Reference Standards
  • Silver Staining
  • Superoxide Dismutase / blood


  • Blood Proteins
  • Buffers
  • Phosphogluconate Dehydrogenase
  • Glucosephosphate Dehydrogenase
  • Glutathione Peroxidase
  • Superoxide Dismutase