Homology of an acrosome-reacted sperm-specific antigen to CD46

J Pharmacobiodyn. 1992 Aug;15(8):455-9. doi: 10.1248/bpb1978.15.455.

Abstract

MH61 is an acrosome-reacted sperm-specific monoclonal antibody. The antibody-coated beads effectively analyze the acrosomal status of human sperm. However, the nature of the antigen was not known. The antigen was purified by immunoprecipitation and SDS-PAGE followed by blotting on PVDF paper. The N-terminal sequence of the antigen was analyzed by an automated protein sequencer. An exactly matching sequence was found in CD46, that is also known as a membrane cofactor protein.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Acrosome / physiology
  • Amino Acid Sequence
  • Antibodies, Monoclonal
  • Antigens, CD / chemistry*
  • Antigens, Surface / chemistry*
  • Antigens, Surface / isolation & purification
  • Electrophoresis, Polyacrylamide Gel
  • Humans
  • Male
  • Membrane Cofactor Protein
  • Membrane Glycoproteins / chemistry*
  • Molecular Sequence Data
  • Precipitin Tests
  • Semen
  • Sequence Homology, Amino Acid
  • Spermatozoa / immunology*

Substances

  • Antibodies, Monoclonal
  • Antigens, CD
  • Antigens, Surface
  • CD46 protein, human
  • Membrane Cofactor Protein
  • Membrane Glycoproteins