Nucleotide sequence analysis of the class G tetracycline resistance determinant from Vibrio anguillarum

Microbiol Immunol. 1992;36(10):1051-60. doi: 10.1111/j.1348-0421.1992.tb02109.x.

Abstract

The nucleotide sequence of the class G tetracycline resistance determinant previously isolated from Vibrio anguillarum has been determined. Two open reading frames of divergent polarity were identified. A resistance gene (tet A) encodes a protein of 393 amino acid residues (deduced molecular mass of 40.9 kDa), and a repressor gene (tet R) encodes a protein consisting of 210 amino acids with a calculated molecular mass of 23.6 kDa. Based on the deduced amino acid sequences, the proteins of tet A(G) and tet R(G) are about 60% homologous with those of RP1/Tn1721 (class A) and pSC101/pBR322 (class C), and about 50% homologous with Tn10 (class B). The relationship of the tet (G) sequence to five known tetracycline resistance determinants (class A to E) is discussed.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Amino Acid Sequence
  • Antiporters*
  • Bacterial Proteins / genetics*
  • Base Sequence
  • DNA, Bacterial
  • Genes, Bacterial
  • Molecular Sequence Data
  • Plasmids
  • Restriction Mapping
  • Sequence Homology, Amino Acid
  • Tetracycline Resistance / genetics*
  • Transcription, Genetic
  • Vibrio / drug effects
  • Vibrio / genetics*

Substances

  • Antiporters
  • Bacterial Proteins
  • DNA, Bacterial
  • tetA protein, Bacteria