Cloning and expression of a heat-inducible hsp70 gene in two species of Hydra which differ in their stress response

Eur J Biochem. 1992 Dec 15;210(3):683-91. doi: 10.1111/j.1432-1033.1992.tb17469.x.

Abstract

A heat-inducible, intron-containing member of the hsp70 gene family has been isolated and characterized in Hydra magnipapillata and Hydra oligactis, two species previously shown [Bosch, T. C. G., Krylow, S. M., Bode, H. R. & Steele, R. E. (1988) Proc. Natl Acad. Sci. USA 85, 7927-7931] to differ in their stress response. The gene, hsp70.1, encodes a 654-amino-acid protein of predicted molecular mass 70 kDa with 78% amino acid identity to Xenopus HSP70. Northern-blot analysis revealed that polyps of H. oligactis accumulate significantly less hsp70.1 mRNA after heat shock than polyps of H. magnipapillata. In nuclear run-off experiments, we found that transcriptional induction of hsp70.1 expression in response to stress is similar in both species. Thus, the previously reported inability of H. oligactis to synthesize heat-shock proteins in response to stress is at least in part due to reduced stability of hsp70.1 mRNA during heat shock.

Publication types

  • Comparative Study
  • Research Support, Non-U.S. Gov't

MeSH terms

  • Amino Acid Sequence
  • Animals
  • Base Sequence
  • Gene Expression
  • Heat-Shock Proteins / biosynthesis
  • Heat-Shock Proteins / genetics*
  • Hot Temperature
  • Humans
  • Hydra / genetics*
  • Introns
  • Molecular Sequence Data
  • Multigene Family*
  • Polymerase Chain Reaction
  • Restriction Mapping
  • Sequence Homology, Amino Acid
  • Transcription, Genetic

Substances

  • Heat-Shock Proteins

Associated data

  • GENBANK/M84018
  • GENBANK/M84019
  • GENBANK/M85175
  • GENBANK/M85176
  • GENBANK/M85177
  • GENBANK/X65179
  • GENBANK/X65180
  • GENBANK/X65181
  • GENBANK/X68290
  • GENBANK/X68291