High-performance liquid chromatographic assay of the methyl and nucleotide metabolites of 6-mercaptopurine: quantitation of red blood cell 6-thioguanine nucleotide, 6-thioinosinic acid and 6-methylmercaptopurine metabolites in a single sample

J Chromatogr. 1992 Nov 27;583(1):83-90. doi: 10.1016/0378-4347(92)80347-s.

Abstract

A reversed-phase high-performance liquid chromatographic assay was developed to quantify intracellular metabolites of the cytotoxic drug 6-mercaptopurine in the human red blood cell. The 6-thioguanine nucleotides, 6-thioinosinic acid and 6-methylmercaptopurine metabolites are measured in a single sample. A similar assay measures the parent thiopurine compounds. The limit of quantitation of the assay is 0.03, 0.03 and 0.12 nmol per 8 x 10(8) red blood cells for the 6-thioguanine nucleotides, 6-thioinosinic acid and the 6-methylmercaptopurine metabolites, respectively.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Chromatography, High Pressure Liquid
  • Erythrocytes / chemistry*
  • Humans
  • Hydrolysis
  • Inosine Monophosphate / analogs & derivatives*
  • Inosine Monophosphate / blood
  • Mercaptopurine / blood*
  • Methylthioinosine / blood*
  • Thionucleotides / blood*

Substances

  • Thionucleotides
  • Inosine Monophosphate
  • Methylthioinosine
  • thioinosinic acid
  • Mercaptopurine