Two new compounds (1,2) were isolated from the ethanolic extract of the leaves of Premna microphylla, together with five known compounds. The structures of compounds 1 and 2 were elucidated as (2S,3S,4R,11E)-2-[(2R)-2-hydroxytetracosanoylaminol-11-octadecene-1,3,4-triol (1) and 1-O-(9Z,12Z, 15Z-octadecatrienoyl)-3-O-[beta-D-galactopyranosyl-(1 --> 6)- O-beta-D-galactopyranosyl-(1 --> 6)-alpha-D-galactopyranosyl] glycerol (2) by means of spectroscopic and chemical methods.