Molecular rescue of tumour-specific T cell receptor idiotype from T cell lymphomas

Br J Haematol. 2004 Mar;124(5):626-8. doi: 10.1111/j.1365-2141.2004.04830.x.

Abstract

The T cell receptor (TCR) idiotype on T cell lymphomas can serve as a vaccine target. To clone the relevant genes, 5' rapid amplification of cDNA ends (RACE) was performed on 13 T cell lymphomas and nine control samples. Two polymerase chain reactions (PCR) were performed for each TCR chain (alpha and beta) and the proportion of the clonal TCR sequence over the total number of TCR sequences was calculated. For alpha, the average proportions were 0.43 vs. 0.05. For beta these were 0.44 and 0.04. The TCR was identified in 10 of 13 lymphoma samples.

Publication types

  • Research Support, Non-U.S. Gov't
  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • Base Sequence
  • Cloning, Molecular
  • Genes, Immunoglobulin / genetics
  • Genes, T-Cell Receptor alpha / genetics*
  • Genes, T-Cell Receptor beta / genetics*
  • Humans
  • Lymphoma, T-Cell / genetics*
  • Polymerase Chain Reaction / methods
  • Receptors, Antigen, T-Cell, alpha-beta / genetics*

Substances

  • Receptors, Antigen, T-Cell, alpha-beta