Imaging calcium concentration dynamics in small neuronal compartments

Sci STKE. 2004 Feb 3;2004(219):pl5. doi: 10.1126/stke.2192004pl5.

Abstract

Calcium and its regulation play central roles diverse physiologic processes. Quantification of calcium concentrations ([Ca2+]) in small neuronal compartments is crucial to understanding Ca2+-dependent signaling. Here, we describe techniques that are optimized for 2-photon imaging of [Ca2+] dynamics in small compartments such as dendrites and dendritic spines.

Publication types

  • Research Support, Non-U.S. Gov't
  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • Animals
  • Calcium Signaling / physiology*
  • Cell Compartmentation / physiology
  • Dendrites / chemistry
  • Dendrites / physiology
  • Hippocampus / cytology
  • Image Processing, Computer-Assisted / instrumentation
  • Image Processing, Computer-Assisted / methods
  • Lasers
  • Microscopy, Fluorescence, Multiphoton / instrumentation
  • Microscopy, Fluorescence, Multiphoton / methods
  • Neurons / chemistry*
  • Neurons / physiology
  • Patch-Clamp Techniques / instrumentation
  • Patch-Clamp Techniques / methods
  • Rats