Rod-like Cholesterol Micelles in Aqueous Solution Studied Using Polarized and Depolarized Dynamic Light Scattering

Biophys J. 1992 Dec;63(6):1455-61. doi: 10.1016/S0006-3495(92)81733-2.


Micelles of cholesterol in aqueous solution have been investigated using polarized and depolarized dynamic light scattering. They are shown to be highly extended and characterized by a narrow size distribution. It is shown that a rod-like model is applicable with length, L = 580 nm. Determination of the rotational diffusion coefficient by analysis of the autocorrelation function gave a value of theta = 150 s-1, which is close to the calculated value for the rod with this dimension. Depolarized dynamic light scattering measurements as a function of angle gave a value of 110 s-1.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Biophysical Phenomena
  • Biophysics
  • Cholesterol / chemistry*
  • Light
  • Micelles
  • Particle Size
  • Scattering, Radiation
  • Solutions
  • Water


  • Micelles
  • Solutions
  • Water
  • Cholesterol