Reconstitution of the T cell receptor alpha beta repertoire in recipients of allogeneic BMT

Bone Marrow Transplant. 1992 Dec;10(6):521-7.


This report describes the qualitative analysis of T cell receptor (TCR) repertoire regeneration in recipients of BMT. RNA samples from patient and control peripheral blood lymphocytes were prepared and tested for the presence of multiple V alpha and V beta transcripts by the polymerase chain reaction. TCR V gene expression was highly diverse within the first 6 months post-transplantation in recipients receiving either T cell-depleted or T cell-replete marrow, and in HLA mismatched as well as matched donor-recipient pairs. The sequencing of TCR message from BMT recipients also demonstrated J gene diversity and apparently normal junctional diversity at the V-J alpha join. Thus, T cell pools in BMT recipients are largely heterogeneous, not mono- or oligoclonal.

Publication types

  • Research Support, Non-U.S. Gov't
  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • Adolescent
  • Base Sequence
  • Bone Marrow Transplantation / immunology*
  • Child
  • DNA / genetics
  • DNA Probes
  • Humans
  • Molecular Sequence Data
  • Polymerase Chain Reaction
  • Receptors, Antigen, T-Cell, alpha-beta / genetics*
  • Tissue Donors
  • Transplantation, Homologous


  • DNA Probes
  • Receptors, Antigen, T-Cell, alpha-beta
  • DNA