Methods for determination of plasmin, antiplasmin and plasminogen by means of substrate S-2251

Haemostasis. 1978;7(2-3):138-45. doi: 10.1159/000214252.


The chromogenic substrate S-2251 (H-D-Val-Leu-Lys-pNA), a selective and sensitive substrate for plasmin activity, has made it possible to develop simple and reproducible methods for the determination of antiplasmin and plasminogen in human plasma. These methods have been optimized and studied in detail and found to be very specific for the respective factors.

MeSH terms

  • Fibrinolysin* / antagonists & inhibitors
  • Fibrinolysin* / standards
  • Humans
  • Plasminogen*
  • Streptokinase / pharmacology


  • Plasminogen
  • Streptokinase
  • Fibrinolysin