Improved high-performance liquid chromatographic determination of ciprofloxacin and its metabolites in human specimens

J Chromatogr. 1992 Nov 6;582(1-2):263-7. doi: 10.1016/0378-4347(92)80331-j.


A simple approach to the quantitation of ciprofloxacin and its three metabolites, M1 (desethylene-ciprofloxacin), M2 (sulfo-ciprofloxacin) and M3 (oxo-ciprofloxacin), in human serum, urine, saliva and sputum is described. This assay allows the parent drug and its metabolites to elute and be resolved in a single chromatogram at 280 nm using a linear gradient. The procedure involved liquid-liquid extraction. Separation was achieved on a C18 reversed-phase column. The limit of detection of ciprofloxacin is 0.05 microgram/ml and that of its three metabolites is 0.25 microgram/ml. This method is sufficiently sensitive for pharmacokinetic studies.

MeSH terms

  • Chromatography, High Pressure Liquid / methods*
  • Ciprofloxacin / analysis*
  • Ciprofloxacin / blood
  • Ciprofloxacin / urine
  • Humans
  • Saliva / chemistry*
  • Sputum / chemistry*


  • Ciprofloxacin