Mis-expression of the CLV3/ESR-like gene CLE19 in Arabidopsis leads to a consumption of root meristem

Gene. 2004 Feb 18;327(1):37-49. doi: 10.1016/j.gene.2003.11.014.

Abstract

Mild heat shock treatment (32 degrees C) of isolated Brassica napus microspores triggers a developmental switch from pollen maturation to embryo formation. This in vitro system was used to identify genes expressed in globular to heart-shape transition embryos. One of the genes isolated encodes a putative extra-cellular protein that exhibits high sequence similarity with the in silico identified CLV3/ESR-related 19 polypeptide from Arabidopsis (AtCLE19) and was therefore named BnCLE19. BnCLE19 is expressed in the primordia of cotyledons, sepals and cauline leaves, and in some pericycle cells in the root maturation zone. Mis-expression of BnCLE19 or AtCLE19 in Arabidopsis under the control of the CaMV 35S promoter resulted in a dramatic consumption of the root meristem, the formations of pin-shaped pistils and vascular islands. These results imply a role of CLE19 in promoting cell differentiation or inhibiting cell division.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Amino Acid Sequence
  • Arabidopsis / genetics*
  • Arabidopsis / growth & development
  • Arabidopsis / ultrastructure
  • Arabidopsis Proteins / genetics*
  • Base Sequence
  • Brassica napus / genetics
  • Cryoelectron Microscopy
  • DNA, Complementary / chemistry
  • DNA, Complementary / genetics
  • DNA, Complementary / isolation & purification
  • DNA, Plant / chemistry
  • DNA, Plant / genetics
  • Gene Expression Regulation, Developmental
  • Gene Expression Regulation, Plant
  • Meristem / genetics*
  • Meristem / growth & development
  • Meristem / ultrastructure
  • Molecular Sequence Data
  • Plant Roots / genetics*
  • Plant Roots / growth & development
  • Plant Roots / ultrastructure
  • Plants, Genetically Modified
  • Pollen / embryology
  • Pollen / genetics
  • Pollen / growth & development
  • Sequence Analysis, DNA
  • Sequence Homology, Amino Acid
  • Temperature

Substances

  • Arabidopsis Proteins
  • CLV3-ESR-related 19 protein, Arabidopsis
  • DNA, Complementary
  • DNA, Plant

Associated data

  • GENBANK/AF343657
  • GENBANK/AF343658