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. 2004 Mar 12;303(5664):1669-74.
doi: 10.1126/science.1094515. Epub 2004 Feb 12.

Evidence of a Pluripotent Human Embryonic Stem Cell Line Derived From a Cloned Blastocyst

Free article

Evidence of a Pluripotent Human Embryonic Stem Cell Line Derived From a Cloned Blastocyst

Woo Suk Hwang et al. Science. .
Free article

Erratum in

  • Science. 2005 Dec 16;310(5755):1769

Retraction in

  • Editorial retraction.
    Kennedy D. Kennedy D. Science. 2006 Jan 20;311(5759):335. doi: 10.1126/science.1124926. Epub 2006 Jan 12. Science. 2006. PMID: 16410485 No abstract available.

Expression of concern in

  • Editorial expression of concern.
    Kennedy D. Kennedy D. Science. 2006 Jan 6;311(5757):36. doi: 10.1126/science.311.5757.36b. Epub 2005 Dec 22. Science. 2006. PMID: 16373531 No abstract available.


Somatic cell nuclear transfer (SCNT) technology has recently been used to generate animals with a common genetic composition. In this study, we report the derivation of a pluripotent embryonic stem (ES) cell line (SCNT-hES-1) from a cloned human blastocyst. The SCNT-hES-1 cells displayed typical ES cell morphology and cell surface markers and were capable of differentiating into embryoid bodies in vitro and of forming teratomas in vivo containing cell derivatives from all three embryonic germ layers in severe combined immunodeficient mice. After continuous proliferation for more than 70 passages, SCNT-hES-1 cells maintained normal karyotypes and were genetically identical to the somatic nuclear donor cells. Although we cannot completely exclude the possibility that the cells had a parthenogenetic origin, imprinting analyses support a SCNT origin of the derived human ES cells.

Comment in

  • Stem cells, redux.
    Kennedy D. Kennedy D. Science. 2004 Mar 12;303(5664):1581. doi: 10.1126/science.303.5664.1581. Science. 2004. PMID: 15016964 No abstract available.
  • Human being redux.
    Gazzaniga MS. Gazzaniga MS. Science. 2004 Apr 16;304(5669):388-9. doi: 10.1126/science.304.5669.388c. Science. 2004. PMID: 15087527 No abstract available.
  • Stem cell research in Korea.
    Song SY. Song SY. Science. 2004 Aug 13;305(5686):944-5; author reply 944-5. doi: 10.1126/science.305.5686.944. Science. 2004. PMID: 15310877 No abstract available.
  • Stem cell research. Korean cloner admits lying about oocyte donations.
    Holden C. Holden C. Science. 2005 Dec 2;310(5753):1402-3. doi: 10.1126/science.310.5753.1402. Science. 2005. PMID: 16322425 No abstract available.
  • Human embryonic stem cells.
    Wilmut I, West MD, Lanza RP, Gearhart JD, Smith A, Colman A, Trounson AO, Campbell KH. Wilmut I, et al. Science. 2005 Dec 23;310(5756):1903. doi: 10.1126/science.1123832. Epub 2005 Dec 13. Science. 2005. PMID: 16352868 No abstract available.

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