Objective: To construct the vector using specific nasopharynx tissue promoter plunc to establish transgeneic mouse model.
Methods: Plunc-EGFP plasmid was digested with XhoI, the purified linearized DNA fragments were recovered by gel extraction and diluted to the final concentration of 4 microg/ml, before introduced into fertilized one-cell mouse eggs by pronuclear microinjection.
Results: Thirteen founder mice were obtained, 12 of which were positive for the integrated EGFP gene as detected by PCR, and 3 were positive shown by Southern blotting.
Conclusion: Specific nasopharynx tissue promoter plunc can effectively induce exogenous gene integration into the mouse genome.