Distribution and characterization of Campylobacter spp. from Russian poultry

J Food Prot. 2004 Feb;67(2):239-45. doi: 10.4315/0362-028x-67.2.239.

Abstract

The distribution of Campylobacter spp. on 13 poultry farms (broiler chicken, quail, pheasant, peacock, and turkey) from eight regions (Vladimir, Vologda, Voronezh, Kaluga, Liptsk, Moscow, Orenburg, and Orel) in Russia was surveyed. Intestinal materials were plated onto Campylobacter-selective medium and plates were incubated microaerobically at 42 degrees C for 24 or 48 h. Identification was based on colonial morphology, microscopic examination, and biochemical tests; latex agglutination assays were used for confirmation. In total, 116 isolates were derived from 370 samples. Isolation rates were similar, regardless of whether the birds were from small or large broiler production farms. Susceptibility of 48 representative (from these production sources) strains of Campylobacter spp. to 38 antimicrobial compounds was determined by disk diffusion assays. All strains tested were sensitive to amikacin, gentamycin, sisomycin, chloramphenicol, imipenem, oleandomycin, erythromycin, azitromycin, and ampicillin. The strains were also sensitive to 100 microg/disk of carbenicillin, fluoroquinolones, and to nitrofurans. Fluoroquinolone sensitivity was most notable and may be related to its limited application in poultry production within Russia. Hippurate and ribosomal RNA gene primers were developed and used to distinguish Campylobacter jejuni and Campylobacter coli and to provide a measure of strain discrimination. The combination of PCR analysis and randomly amplified polymorphic DNA (RAPD) typing were conducted for selected isolates. The various poultry species and the different locations yielded Campylobacter isolates with discrete randomly amplified polymorphic DNA patterns. The distribution and substantial diversity of Campylobacter spp. isolates appears similar to that previously reported in other countries.

MeSH terms

  • Animals
  • Anti-Bacterial Agents / pharmacology*
  • Campylobacter / drug effects*
  • Campylobacter / genetics
  • Campylobacter / isolation & purification*
  • Chickens / microbiology
  • Colony Count, Microbial
  • DNA, Bacterial / analysis
  • Drug Resistance, Bacterial*
  • Food Contamination / analysis
  • Food Microbiology
  • Genetic Variation*
  • Microbial Sensitivity Tests
  • Polymorphism, Restriction Fragment Length
  • Poultry / microbiology*
  • Prevalence
  • Quail / microbiology
  • Russia / epidemiology
  • Species Specificity
  • Turkeys / microbiology

Substances

  • Anti-Bacterial Agents
  • DNA, Bacterial