Characterization and quantification of anthocyanins and polyphenolics in bluehHoneysuckle (Lonicera caerulea L.)

J Agric Food Chem. 2004 Feb 25;52(4):848-52. doi: 10.1021/jf030509o.


Anthocyanins and phenolics of 10 blue honeysuckle (Lonicera caerulea L.) genotypes were characterized and quantified by HPLC-DAD. Peak assignments were confirmed by low-resolution electrospray mass spectrometry. Six anthocyanins were detected with the major peak identified as cyanidin 3-glucoside. Five additional anthocyanins were characterized as cyanidin 3,5-diglucoside, cyanidin 3-rutinoside, pelargonidin 3-glucoside, peonidin 3-glucoside, and peonidin 3-rutinoside. Four polyphenolics were identified as chlorogenic acid, neochlorogenic acid, quercetin 3-rutinoside, and quercetin 3-glucoside. Two additional unidentified phenolics were characterized as flavonol and hydroxycinnamic derivatives based on UV-vis spectra. Hydroxycinnamate levels ranged from 30.4 to 156.2 mg/100 g, whereas the flavonol content ranged from 12.6 to 32.8 mg/100 g. The L. caerulea subspecies boczkarnikovae contained the highest amounts of hydroxycinnamic derivatives and flavonols.

MeSH terms

  • Anthocyanins / analysis*
  • Chromatography, High Pressure Liquid
  • Coumaric Acids / analysis
  • Flavonoids / analysis*
  • Flavonols / analysis
  • Lonicera / chemistry*
  • Phenols / analysis*
  • Polyphenols
  • Spectrometry, Mass, Electrospray Ionization


  • Anthocyanins
  • Coumaric Acids
  • Flavonoids
  • Flavonols
  • Phenols
  • Polyphenols