Complete 1H and 13C NMR assignments of two phytosterols from roots of Piper nigrum

Magn Reson Chem. 2004 Mar;42(3):355-9. doi: 10.1002/mrc.1347.

Abstract

Using modern NMR techniques, including DQF-COSY, NOESY, HETCOR and HMBC, the full assignments of all 1H and 13C chemical shifts were made for stigmastane-3,6-dione and stigmast-4-ene-3,6-dione, isolated from the roots of Piper nigrum (Piperaceae). Their stereochemistry was resolved by a combination of 2D NOESY data and analysis of coupling constants. The two compounds were isolated from the genus Piper for the first time.

MeSH terms

  • Chloroform
  • Cholestenones / chemistry*
  • Magnetic Resonance Spectroscopy
  • Models, Molecular
  • Phytosterols / chemistry*
  • Piper nigrum / chemistry*
  • Plant Roots / chemistry
  • Solvents

Substances

  • Cholestenones
  • Phytosterols
  • Solvents
  • stigmastane-3,6-dione
  • stigmast-4-ene-3,6-dione
  • Chloroform