Identification of a novel thioredoxin-related protein, PC-TRP, which is preferentially expressed in plasma cells

Eur J Immunol. 2004 Jan;34(1):137-46. doi: 10.1002/eji.200324225.


Using cDNA subtraction we have identified a previously unknown cDNA, termed plasma cell thioredoxin-related protein (PC-TRP), which was overexpressed in plasma cells in vitro. The full-length 2636-bp PC-TRP cDNA contained an open reading frame coding for 417 amino acids. The cDNA sequence was mapped to a region on mouse chromosome 13 and the PC-TRP gene consists of ten exons distributed over a 28-kb region. The PC-TRP was found to contain three atypical thioredoxin domains and showed functional thioredoxin activity in vitro. A more detailed analysis revealed that PC-TRP was expressed at a high level in plasma cells, both at the mRNA and protein level, while present at very low levels in all other cells and tissues examined. Immunohistological analysis revealed that PC-TRP was expressed at high levels in all plasma cells isolated from bone marrow or lamina propria, irrespective of their Ig isotype. In the spleen, however, 60% of the IgG-expressing plasma cells showed only a dull staining at day 14 after immunization, while >90% of the IgG-secreting cells in the spleen expressed PC-TRP at day 21 after immunization. In addition, an IgG-secreting, PC-TRPdull plasma cell population could also be observed after B cell activation in vitro.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Amino Acid Sequence
  • Animals
  • Cell Differentiation / genetics
  • DNA, Complementary
  • Gene Expression Profiling
  • Immunohistochemistry
  • Mice
  • Mice, Inbred C57BL
  • Molecular Sequence Data
  • Plasma Cells / metabolism*
  • Sequence Analysis, DNA
  • Spleen / metabolism
  • Thioredoxins / genetics*
  • Thioredoxins / metabolism


  • DNA, Complementary
  • PC-TRP protein, mouse
  • Thioredoxins