Raji B cells, misidentified as THP-1 cells, stimulate DC-SIGN-mediated HIV transmission

Virology. 2004 Jan 5;318(1):17-23. doi: 10.1016/j.virol.2003.09.028.


A number of studies examining interactions of dendritic cell (DC)-specific ICAM-3 grabbing nonintegrin (DC-SIGN) with viral pathogens have relied on monocytic transfectants as models for primary DCs. Here we show that the presumed "THP-1" monocytic cells used in these studies are instead Raji B cells. Moreover, we demonstrate that true THP-1 cells do not support DC-SIGN-mediated HIV-1 transmission, whereas human B cell lines efficiently enhance this process. These data indicate that there are features common to B cells and DCs that facilitate transmission of HIV-1 and provide new insights toward the mechanism of DC-SIGN-mediated HIV-1 transmission.

Publication types

  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • B-Lymphocytes / virology*
  • Cell Adhesion Molecules / genetics
  • Cell Adhesion Molecules / metabolism*
  • Cell Line
  • HIV Infections / transmission*
  • HIV-1 / pathogenicity*
  • Humans
  • Lectins, C-Type / genetics
  • Lectins, C-Type / metabolism*
  • Monocytes / virology*
  • Receptors, Cell Surface / genetics
  • Receptors, Cell Surface / metabolism*


  • Cell Adhesion Molecules
  • DC-specific ICAM-3 grabbing nonintegrin
  • Lectins, C-Type
  • Receptors, Cell Surface