Characterization of two kinases involved in thiamine pyrophosphate and pyridoxal phosphate biosynthesis in Bacillus subtilis: 4-amino-5-hydroxymethyl-2methylpyrimidine kinase and pyridoxal kinase

J Bacteriol. 2004 Mar;186(5):1571-3. doi: 10.1128/JB.186.5.1571-1573.2004.


Two Bacillus subtilis genes encoding two proteins (currently annotated ThiD and YjbV) were overexpressed and characterized. YjbV has 4-amino-5-hydroxymethyl-2-methylpyrimidine and 4-amino-5-hydroxymethyl-2-methylpyrimidine pyrophosphate kinase activity and should be reannotated ThiD, and B. subtilis ThiD has pyridoxine, pyridoxal, and pyridoxamine kinase activity and should be reannotated PdxK.

Publication types

  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • Bacillus subtilis / enzymology*
  • Pyridoxal Kinase / metabolism*
  • Pyridoxal Phosphate / biosynthesis*
  • Pyrimidines / metabolism
  • Substrate Specificity
  • Thiamine Pyrophosphate / biosynthesis*


  • 4-amino-5-hydroxymethyl-2-methylpyrimidine
  • Pyrimidines
  • Pyridoxal Phosphate
  • Pyridoxal Kinase
  • Thiamine Pyrophosphate