Quantitative trait locus analysis of plasma cholesterol and triglyceride levels in KK x RR F2 mice

Biochem Genet. 2003 Oct;41(9-10):325-41. doi: 10.1023/b:bigi.0000006033.99978.8b.

Abstract

A previous quantitative trait locus (QTL) study on hyperlipidemia in C57BL/6J x KK-Ay/a F2 mice identified three significant cholesterol QTLs (Cq1 and Cq2 on chromosome 1, and Cq3 on chromosome 3), and a suggestive triglyceride QTL on chromosome 9. An alternative analysis of this study identified a novel cholesterol QTL on chromosome 9 (Cq4), and a significant triglyceride QTL on chromosome 9 (Tgq1). In the present study, QTL analysis was performed on KK x RR F2 mice. A significant cholesterol QTL (Cq5, lod score 5.6) was identified on chromosome 9, and a significant triglyceride QTL (Tgq2, lod score 4.7) was identified on chromosome 8. The Cq5 locus was mapped to a region similar to the Cq4 locus. On the other hand, the Tgq2 locus overlapped with the QTL region responsible for glucose intolerance (Giq1) that was identified in a previous study. The results suggest that a different combination of QTLs is involved in the trait when a different counterpart strain is used. Identification of distinct, but related traits in an identical chromosomal region will facilitate revealing the responsible gene.

Publication types

  • Comparative Study
  • Research Support, Non-U.S. Gov't

MeSH terms

  • Alleles
  • Amino Acid Sequence
  • Amino Acid Substitution
  • Animals
  • Cholesterol / blood*
  • Cholesterol / genetics*
  • Chromosome Mapping / methods
  • Chromosomes / genetics
  • DNA, Complementary / genetics
  • Female
  • Genetic Markers / genetics
  • Glucose Intolerance / genetics
  • Male
  • Mice
  • Mice, Inbred C57BL
  • Mice, Inbred Strains
  • Microsatellite Repeats / genetics
  • Open Reading Frames / genetics
  • Point Mutation
  • Quantitative Trait Loci / genetics*
  • Triglycerides / blood*
  • Triglycerides / genetics*

Substances

  • DNA, Complementary
  • Genetic Markers
  • Triglycerides
  • Cholesterol