Characterization of phytochelatin synthase-like protein encoded by alr0975 from a prokaryote, Nostoc sp. PCC 7120

Biochem Biophys Res Commun. 2004 Mar 12;315(3):751-5. doi: 10.1016/j.bbrc.2004.01.122.


Phytochelatins (PCs) are well known as the heavy metal-detoxifying peptides in higher plants, eukaryotic algae, fungi, and nematode. In contrast, neither PCs nor PC synthase genes have ever been identified in any prokaryotes. The genome sequences for the cyanobacterium Nostoc sp. PCC 7120 were recently completed and allowed us to identify a gene encoding a PC synthase-like protein, termed alr0975. The predicted product of alr0975 contains the conserved N-terminal domain but not the variable C-terminal domain found in eukaryotic PC synthases. The recombinant alr0975 protein strongly catalyzed the first step of PC synthesis, in which glutathione (GSH) is converted to gamma-glutamylcysteine (gamma-EC), although the protein only weakly catalyzed the second step of PC synthesis, namely the transfer of gamma-EC moiety to an acceptor GSH molecule to form PC(2). These results suggest alr0975 protein may be a more primitive form of the PC synthases found in eukaryotes.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Amino Acid Sequence
  • Aminoacyltransferases / chemistry
  • Aminoacyltransferases / genetics*
  • Aminoacyltransferases / metabolism
  • Cyanobacteria / enzymology*
  • Cyanobacteria / genetics*
  • DNA Primers / genetics
  • Dipeptides / metabolism
  • Escherichia coli / genetics
  • Escherichia coli / metabolism
  • Glutathione
  • Metalloproteins / metabolism
  • Molecular Sequence Data
  • Phytochelatins
  • Recombinant Proteins / genetics
  • Recombinant Proteins / metabolism
  • Sequence Alignment
  • Sequence Homology, Amino Acid
  • Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization / methods


  • DNA Primers
  • Dipeptides
  • Metalloproteins
  • Recombinant Proteins
  • Phytochelatins
  • Aminoacyltransferases
  • glutathione gamma-glutamylcysteinyltransferase
  • Glutathione
  • gamma-glutamylcysteine