Differential binding efficiency between the envelope protein of Japanese encephalitis virus variants and heparan sulfate on the cell surface

J Med Virol. 2004 Apr;72(4):618-24. doi: 10.1002/jmv.20025.


Japanese encephalitis (JE) virus infects a number of host cells, either mosquitoes or vertebrates, in nature. The viral envelope (E) protein is known to interact with molecule(s) on the cell membrane during the early stage of virus infection. In this study, two sets of virus variants including T1P1-L4/T1P1-S1 and CJN-L1/CJN-S1 derived from two strains (T1P1 and CJN) of the JE virus were used to evaluate the effects of genomic variations on virus entry. Each set of virus variant (T1P1-L4/T1P1-S1 or CJN-L1/CJN-S1) possessed a single amino acid variation in the E protein. The variation of Glu/Lys at E-306 was found between T1P1-L4 and T1P1-S1 whereas the same variation at E-138 was seen between CJN-L1 and CJN-S1. The results showed that heparan sulfate (HS) differentially expressed on the surface of different types of host cells was essential for JE virus infection as shown in an evident difference in attachment efficiency between CHO-K1 cells and its mutant with defects in GAG biosynthesis. Furthermore, differential interaction of heparin with the envelope protein of JE virus variants implies the significance of virus mutations (especially Lys for E-138 and/or E306 in this case) that are rather likely involved in determining efficiencies of viral attachment, penetration, and eventual infection.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • CHO Cells
  • Cell Line
  • Cell Membrane / virology
  • Cricetinae
  • Encephalitis Virus, Japanese / genetics
  • Encephalitis Virus, Japanese / growth & development
  • Encephalitis Virus, Japanese / metabolism*
  • Genetic Variation*
  • Heparitin Sulfate / biosynthesis
  • Heparitin Sulfate / genetics
  • Heparitin Sulfate / metabolism*
  • Membrane Glycoproteins / genetics*
  • Membrane Glycoproteins / metabolism*
  • Molecular Sequence Data
  • Mutation, Missense
  • Protein Binding
  • Receptors, Virus / physiology
  • Viral Envelope Proteins / genetics*
  • Viral Envelope Proteins / metabolism*
  • Viral Plaque Assay


  • Membrane Glycoproteins
  • Receptors, Virus
  • Viral Envelope Proteins
  • glycoprotein E, Japanese encephalitis virus
  • Heparitin Sulfate

Associated data

  • GENBANK/AF303791
  • GENBANK/AF303792
  • GENBANK/AF303793
  • GENBANK/AF303794