Mutations in TAP genes are common in cervical carcinomas

Gynecol Oncol. 2004 Mar;92(3):914-21. doi: 10.1016/j.ygyno.2003.11.037.

Abstract

Objective: To determine whether squamous cervical cancers exhibit mutations or deletions in MHC class I genes or transport-associated protein (TAP) genes.

Methods: Polymerase chain reaction based protocols were used to examine HLA class I and TAP genes in a panel of cervical tumours, using DNA from corresponding blood samples as controls. SSP-PCR protocols were similarly used for examination of all TAP alleles in tumour and blood samples.

Results: In a series of cervical carcinomas, 7 of 27 (26%) exhibited mutations in HLA-A genes, while 12 of 23 (52%) exhibited mutations in TAP genes. HLA gene mutations were detected in 2 of 14 CIN2-3 lesions, and TAP gene mutations in none of 14, a frequency significantly less than observed in the cervical carcinoma samples (P<0.01). The TAP 2A/2B heterozygous genotype was observed with increased frequency in patients with cervical cancer compared to population controls (P<0.02).

Conclusion: These data suggest that TAP genes may be relevant to evolution of cervical cancer from precursor lesions.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • ATP-Binding Cassette Transporters
  • Alleles
  • Carcinoma, Squamous Cell / blood
  • Carcinoma, Squamous Cell / genetics*
  • Carcinoma, Squamous Cell / immunology*
  • Carcinoma, Squamous Cell / pathology
  • Cervical Intraepithelial Neoplasia / blood
  • Cervical Intraepithelial Neoplasia / genetics*
  • Cervical Intraepithelial Neoplasia / immunology*
  • Cervical Intraepithelial Neoplasia / pathology
  • Female
  • Gene Deletion
  • HLA-A Antigens / genetics
  • HLA-A Antigens / immunology
  • Histocompatibility Antigens Class I / genetics*
  • Histocompatibility Antigens Class I / immunology
  • Humans
  • Mutation
  • Neoplasm Staging
  • Uterine Cervical Neoplasms / blood
  • Uterine Cervical Neoplasms / genetics*
  • Uterine Cervical Neoplasms / immunology*
  • Uterine Cervical Neoplasms / pathology

Substances

  • ATP-Binding Cassette Transporters
  • HLA-A Antigens
  • Histocompatibility Antigens Class I
  • transporter associated with antigen processing (TAP)