Whole blood and mononuclear cell glutathione response to dietary whey protein supplementation in sedentary and trained male human subjects

Int J Food Sci Nutr. 2004 Mar;55(2):131-41. doi: 10.1080/096374080410001666504.

Abstract

Sedentary male subjects (n=9) on a controlled diet were fed two doses (0.8 or 1.6 g/kg body mass) of a whey protein isolate (WPI), in addition to an isocaloric placebo; blood samples were drawn over a 4-h period and glutathione concentration determined. There was no effect of the supplementation at either level over the 4-h sampling period. The effects of a WPI supplemented diet on glutathione concentrations in whole blood as well as peripheral mononuclear cell populations were also investigated over a 6-week period in male subjects (n=18) involved in arduous aerobic training; blood was collected prior to and following a 40 km simulated cycling trial. The aerobic training period resulted in significantly lower glutathione concentrations in whole blood, an effect that was mitigated by WPI supplementation. A significant increase in mononuclear cell glutathione was also observed in subjects receiving the WPI supplement following the 40 km simulated cycling trial.

Publication types

  • Clinical Trial
  • Randomized Controlled Trial
  • Research Support, Non-U.S. Gov't

MeSH terms

  • Adult
  • Amino Acids / analysis
  • Bicycling / physiology
  • Dietary Proteins / pharmacology*
  • Dietary Supplements*
  • Dose-Response Relationship, Drug
  • Exercise / physiology
  • Glutathione / blood*
  • Humans
  • Leukocytes, Mononuclear / metabolism*
  • Male
  • Milk Proteins / pharmacology*
  • Whey Proteins

Substances

  • Amino Acids
  • Dietary Proteins
  • Milk Proteins
  • Whey Proteins
  • Glutathione