Novel bifunctional probe for radioisotope-free photoaffinity labeling: compact structure comprised of photospecific ligand ligation and detectable tag anchoring units

Org Biomol Chem. 2004 Mar 7;2(5):637-41. doi: 10.1039/b316221d. Epub 2004 Feb 4.


A novel method for radioisotope-free photoaffinity labeling was developed, in which a bifunctional ligand is connected to a target protein by activation of a photoreactive group, such as an aromatic azido or 3-trifluoromethyl-3H-diazirin-3-yl group, and identification of the ligated product is achieved by anchoring of a detectable tag through the Staudinger-Bertozzi reaction with an alkyl azido moiety that survives photolysis. The chemical ground of this method was confirmed using model compounds with the bifunctional group under photoirradiation in the presence of trapping agents for reactive intermediates. The utility of the method has been demonstrated by specific labeling of the catalytic portion of human HMG-CoA reductase.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Azides / chemistry
  • Electrophoresis, Polyacrylamide Gel / methods
  • Humans
  • Hydroxymethylglutaryl CoA Reductases / chemistry
  • Hydroxymethylglutaryl CoA Reductases / radiation effects
  • Ligands
  • Light
  • Models, Molecular
  • Molecular Structure
  • Photoaffinity Labels / chemistry*
  • Photolysis
  • Protein Conformation
  • Proteins / chemistry*
  • Proteins / radiation effects
  • Sensitivity and Specificity


  • Azides
  • Ligands
  • Photoaffinity Labels
  • Proteins
  • Hydroxymethylglutaryl CoA Reductases