In vitro expression and pharmacology of the 5-HT7-like receptor present in the mosquito Aedes aegypti tracheolar cells and hindgut-associated nerves

Insect Mol Biol. 2003 Dec;12(6):561-9. doi: 10.1046/j.1365-2583.2003.00441.x.

Abstract

We have previously reported the cloning of a 5-hydroxytryptamine receptor (Aedes 5-HT7-like receptor) from adult Aedes aegypti. For functional expression of the Aedes 5-HT7-like receptor, CHO-K1 cells were stably transfected with a receptor expression construct, pC5-HT7. The Aedes 5-HT7-like receptor positively coupled to Gs protein, increasing intracellular cAMP in response to 5-HT; adenylyl cyclase activity was induced in a concentration-dependent, saturable manner. Only 5-HT, and not octopamine, dopamine or tyramine, caused the induction of cAMP. At 10 nM 5-HT a weak synergism was observed between octopamine and 5-HT. Other known agonists of the mammalian 5-HT7 receptor were tested. Their order of potency was: 5-HT >> 5-CT = 8-OH-DPAT >> pimozide. This is the first report on the functional expression of a mosquito neurohormone receptor.

Publication types

  • Comparative Study
  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • Adenylyl Cyclases / metabolism
  • Aedes / genetics*
  • Animals
  • CHO Cells
  • Cricetinae
  • Cricetulus
  • Cyclic AMP / biosynthesis
  • DNA Primers
  • Dopamine
  • Gene Expression*
  • In Vitro Techniques
  • Octopamine
  • Receptors, Serotonin / genetics*
  • Receptors, Serotonin / metabolism*
  • Reverse Transcriptase Polymerase Chain Reaction
  • Serotonin / metabolism
  • Transfection
  • Tyramine

Substances

  • DNA Primers
  • Receptors, Serotonin
  • serotonin 7 receptor
  • Octopamine
  • Serotonin
  • Cyclic AMP
  • Adenylyl Cyclases
  • Dopamine
  • Tyramine