The metNPQ operon of Bacillus subtilis encodes an ABC permease transporting methionine sulfoxide, D- and L-methionine

Res Microbiol. 2004 Mar;155(2):80-6. doi: 10.1016/j.resmic.2003.11.008.


The Bacillus subtilis yusCBA operon, which encodes an ABC-type transporter, contains an S-box motif in its promoter region. We showed that the expression of these genes is repressed via the S-box system when methionine is available. The YusCB proteins are involved in the transport of both d- and l-methionine but also methionine sulfoxide. A yusCB mutant is unable to grow in the presence of 5 microM l-methionine or 100 microM methionine sulfoxide, while it grows similarly to the wild type with 100 microM l-methionine and 1 mM methionine sulfoxide. Other uptake systems are therefore present for these two compounds. In contrast, the Yus ABC transporter corresponds to the sole d-methionine uptake system. We propose to rename yusC, yusB and yusA as metN, metP and metQ, respectively.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • ATP-Binding Cassette Transporters / genetics
  • ATP-Binding Cassette Transporters / metabolism*
  • Adenosine Triphosphatases / metabolism
  • Amino Acid Transport Systems / genetics
  • Amino Acid Transport Systems / metabolism
  • Bacillus subtilis / genetics
  • Bacillus subtilis / growth & development
  • Bacillus subtilis / metabolism*
  • Carrier Proteins / metabolism
  • Escherichia coli Proteins / metabolism
  • Methionine / analogs & derivatives*
  • Methionine / metabolism*
  • Operon / genetics


  • ATP-Binding Cassette Transporters
  • Amino Acid Transport Systems
  • Carrier Proteins
  • Escherichia coli Proteins
  • MetQ protein, E coli
  • Methionine
  • Adenosine Triphosphatases
  • MetN protein, E coli
  • methionine sulfoxide