Moving fluid with bacterial carpets

Biophys J. 2004 Mar;86(3):1863-70. doi: 10.1016/S0006-3495(04)74253-8.

Abstract

We activated a solid-fluid interface by attaching flagellated bacteria to a solid surface. We adsorbed swarmer cells of Serratia marcescens to polydimethylsiloxane or polystyrene. The cell bodies formed a densely packed monolayer while their flagella continued to rotate freely. Motion of the fluid close to an extended flat surface, visualized with tracer beads, was dramatically enhanced compared to the motion farther away. The tracer beads revealed complex ever-changing flow patterns, some linear (rivers), others rotational (whirlpools). Typical features of this flow were small (tens of micro m) and reasonably stable (many minutes). The surface performed active mixing equivalent to diffusion with a coefficient of 2 x 10(-7) cm(2)/s. We call these flat constructs "bacterial carpets". When attached to polystyrene beads or to fragments of polydimethylsiloxane, the bacteria generated both translation and rotation. We call these constructs "auto-mobile beads" or "auto-mobile chips". Given the size and strength of the flow patterns near the carpets, the motion must be generated by small numbers of coordinated flagella. We should be able to produce larger and longer-range effects by increasing coordination.

Publication types

  • Research Support, Non-U.S. Gov't
  • Research Support, U.S. Gov't, Non-P.H.S.

MeSH terms

  • Biofilms
  • Cell Adhesion / physiology
  • Cell Culture Techniques / methods
  • Flagella / physiology*
  • Microfluidics / methods*
  • Molecular Motor Proteins / physiology*
  • Molecular Motor Proteins / ultrastructure*
  • Motion
  • Serratia marcescens / cytology*
  • Serratia marcescens / physiology*

Substances

  • Molecular Motor Proteins