Validation of 8-isoprostane and prostaglandin E(2) measurements in exhaled breath condensate

Inflamm Res. 2003 Dec;52(12):502-7. doi: 10.1007/s00011-003-1212-6. Epub 2003 Nov 13.


Objective: To qualitatively validate radioimmunoassays for 8-isoprostane and prostaglandin (PG) E(2) in exhaled breath condensate.

Subjects: Twenty-two subjects with different lung diseases attended the outpatient clinic on one occasion for exhaled breath condensate collection.

Methods: Samples were pooled together and purified by reverse phase high performance liquid chromatography (RP-HPLC). The eluted fractions were assayed for 8-isoprostane-like immunoreactivity and PGE(2)-like immunoreactivity by radioimmunoassays. In addition, simultaneous measurements of exhaled breath condensate unextracted samples with two anti-8-isoprostane and anti-PGE(2) sera with different cross-reactivity were performed.

Results: A single peak of 8-isoprostane-like immunoreactivity and PGE(2)-like immunoreactivity co-eluting with 8-isoprostane (retention time: 13 min) and PGE(2) (retention time: 21 min) standards, respectively, was identified by radioimmunoassays. Testing with two different antisera showed similar results for both 8-isoprostane-like immunoreactivity (limits of agreement = 4.5 pg/ml and - 4.1 pg/ml, n = 12) and PGE(2)-like immunoreactivity (limits of agreement = 6.1 pg/ ml and - 6.1 pg/ml, n = 12).

Conclusion: This study provides evidence for the specificity of the radioimmunoassays for 8-isoprostane and PGE(2) in exhaled breath condensate. This is critical for proposing these markers as a non-invasive way for monitoring airway inflammation.

Publication types

  • Clinical Trial
  • Research Support, Non-U.S. Gov't

MeSH terms

  • Breath Tests / methods*
  • Chromatography, High Pressure Liquid
  • Dinoprostone / analysis*
  • Dinoprostone / blood
  • Female
  • Humans
  • Isoprostanes / analysis*
  • Isoprostanes / blood
  • Lung Diseases / blood
  • Lung Diseases / diagnosis*
  • Lung Diseases / metabolism
  • Male
  • Middle Aged
  • Radioimmunoassay
  • Reproducibility of Results
  • Respiration


  • Isoprostanes
  • Dinoprostone