Abstract
A novel integral membrane lipoprotein, Wza, from Escherichia coli serotype O9a:K30 has been purified and crystallized. Wza is required for the surface expression of the serotype K30 group 1 capsular polysaccharide of E. coli; closely related homologues are found in other bacteria that produce extracellular polysaccharides. The Wza crystals belong to the orthorhombic space group P2(1)2(1)2(1), with unit-cell parameters a = 94.6, b = 215.5, c = 218.5 A. A data set to 3.0 A with 99.8% completeness and an R(merge) of 10.5% has been collected from a single crystal.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Antigens, Bacterial / biosynthesis
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Antigens, Surface / biosynthesis
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Bacterial Outer Membrane Proteins / chemistry*
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Bacterial Outer Membrane Proteins / metabolism
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Crystallization
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Crystallography, X-Ray
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Escherichia coli / enzymology*
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Escherichia coli Proteins / chemistry*
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Escherichia coli Proteins / metabolism
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Polysaccharides / metabolism
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Structural Homology, Protein
Substances
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Antigens, Bacterial
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Antigens, Surface
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Bacterial Outer Membrane Proteins
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Escherichia coli Proteins
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K antigens
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Polysaccharides
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Wza protein, E coli