Abstract
The EutD protein of Salmonella enterica is homologous to the catalytic domain of the phosphotransacetylase (Pta) enzyme. The Pta-like activity level of the EutD enzyme compared favorably to that of other Pta enzymes. High-pressure liquid chromatography and mass spectrometry verified that acetyl-coenzyme A was the product of the reaction. The EutD protein restored growth of an S. enterica pta strain on acetate as the source of carbon and energy.
Publication types
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Research Support, U.S. Gov't, P.H.S.
MeSH terms
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Acetates / metabolism
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Bacterial Proteins / genetics*
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Bacterial Proteins / metabolism
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Chromatography, High Pressure Liquid
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Culture Media
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Mass Spectrometry
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Phosphate Acetyltransferase / genetics*
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Phosphate Acetyltransferase / metabolism*
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Salmonella enterica / enzymology
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Salmonella enterica / genetics*
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Salmonella enterica / growth & development
Substances
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Acetates
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Bacterial Proteins
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Culture Media
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Phosphate Acetyltransferase