The eutD Gene of Salmonella Enterica Encodes a Protein With Phosphotransacetylase Enzyme Activity

J Bacteriol. 2004 Mar;186(6):1890-2. doi: 10.1128/jb.186.6.1890-1892.2004.

Abstract

The EutD protein of Salmonella enterica is homologous to the catalytic domain of the phosphotransacetylase (Pta) enzyme. The Pta-like activity level of the EutD enzyme compared favorably to that of other Pta enzymes. High-pressure liquid chromatography and mass spectrometry verified that acetyl-coenzyme A was the product of the reaction. The EutD protein restored growth of an S. enterica pta strain on acetate as the source of carbon and energy.

Publication types

  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • Acetates / metabolism
  • Bacterial Proteins / genetics*
  • Bacterial Proteins / metabolism
  • Chromatography, High Pressure Liquid
  • Culture Media
  • Mass Spectrometry
  • Phosphate Acetyltransferase / genetics*
  • Phosphate Acetyltransferase / metabolism*
  • Salmonella enterica / enzymology
  • Salmonella enterica / genetics*
  • Salmonella enterica / growth & development

Substances

  • Acetates
  • Bacterial Proteins
  • Culture Media
  • Phosphate Acetyltransferase