Probity: A Protein Identification Algorithm With Accurate Assignment of the Statistical Significance of the Results

J Proteome Res. Jan-Feb 2004;3(1):32-6. doi: 10.1021/pr034048y.


An algorithm for protein identification based on mass spectrometric proteolytic peptide mapping and genome database searching is presented. The algorithm ranks database proteins based on direct calculation of the probability of random matching and assigns the statistical significance to each result. We investigate the performance of the algorithm by simulation and show that the algorithm responds to random data in the desired manner and that the statistical significance computed indicates the risk that a particular identification result is false.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Algorithms*
  • Computer Simulation
  • Databases, Protein
  • Models, Statistical
  • Proteins / analysis*


  • Proteins