Impact of staphylococcal protease expression on the outcome of infectious arthritis

Ann-Marie Calander; Ing-Marie Jonsson; Anna Kanth; Staffan Arvidsson; Lindsey Shaw; Simon J Foster; Andrej Tarkowski

doi:10.1016/j.micinf.2003.10.015

Impact of staphylococcal protease expression on the outcome of infectious arthritis

Microbes Infect. 2004 Feb;6(2):202-6. doi: 10.1016/j.micinf.2003.10.015.

Authors

Ann-Marie Calander¹, Ing-Marie Jonsson, Anna Kanth, Staffan Arvidsson, Lindsey Shaw, Simon J Foster, Andrej Tarkowski

Affiliation

¹ Department of Rheumatology and Inflammation Research, University of Gothenburg, Guldhedsgatan 10A, 41346 Göteborg, Sweden. ann-marie.calander@rheuma.gu.se

PMID: 14998519
DOI: 10.1016/j.micinf.2003.10.015

Abstract

The exoproteases of Staphylococcus aureus have been proposed as virulence factors during S. aureus infections. To investigate this, we used the wild-type S. aureus strain 8325-4 and its mutants devoid of aureolysin, serine protease, and cysteine protease, respectively, in a well-established model of septic arthritis in mice. The inactivation of the exoprotease genes did not affect the frequency or the severity of joint disease. We conclude that in the model of haematogenously spread staphylococcal arthritis, the bacterial proteases studied do not act as virulence factors.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Animals
Arthritis, Infectious / microbiology*
Arthritis, Infectious / pathology
Cysteine Endopeptidases / genetics*
Disease Models, Animal
Female
Gene Expression Regulation, Enzymologic
Genes, Bacterial
Mice
Serine Endopeptidases / genetics
Serine Endopeptidases / metabolism
Serine Endopeptidases / toxicity*
Staphylococcal Infections / microbiology*
Staphylococcus aureus / enzymology
Staphylococcus aureus / genetics
Staphylococcus aureus / pathogenicity*
Virulence Factors / toxicity

Substances

Virulence Factors
Serine Endopeptidases
Cysteine Endopeptidases