Alamethicin-induced pore formation in biological membranes

Gen Physiol Biophys. 1992 Feb;11(1):49-58.

Abstract

The effects of alamethicin on the membrane barrier function of rabbit erythrocytes, human platelets and sarcoplasmic reticulum vesicles, as well as on that of brain microsomes and liver mitochondria of the rat were compared. An upset of the barrier function was observed for plasma membranes of brain microsomes as well as for erythrocyte and platelet membranes at alamethicin concentrations ranging between 25-80 micrograms/ml. The membrane barrier functions of sarcoplasmic reticulum vesicles, of endoplasmic reticulum vesicles of rat brain microsomes, and of liver mitochondria were disturbed at 3-7 micrograms/ml alamethicin. The different sensitivities of plasma and intracellular membranes to alamethicin were supposed to be due to the presence of considerable quantities of cholesterol in plasma membranes as well as to peculiarities of their protein compositions.

Publication types

  • Comparative Study

MeSH terms

  • Alamethicin / pharmacology*
  • Animals
  • Cell Membrane / drug effects*
  • Cell Membrane / metabolism
  • Cell Membrane Permeability / drug effects
  • Cholesterol / metabolism
  • Humans
  • In Vitro Techniques
  • Intracellular Membranes / drug effects*
  • Intracellular Membranes / metabolism
  • Membrane Lipids / metabolism
  • Membrane Proteins / metabolism
  • Rabbits
  • Rats

Substances

  • Membrane Lipids
  • Membrane Proteins
  • Alamethicin
  • Cholesterol