Effect of non-digestible gluco-oligosaccharides on glucose sensitivity in high fat diet fed mice

J Physiol Biochem. 2003 Sep;59(3):169-73. doi: 10.1007/BF03179912.


Non digestible dietary carbohydrates have been reported to modify lipaemia and post-prandial glycaemia and insulinaemia. The aim of this study was to investigate the effect of a non-digestible gluco-oligosaccharides (GOS) diet on glucose, insulin, triglycerides and free fatty acid blood levels and glucose sensitivity in high fat diet fed mice (a high fat diet composed of 45% fat, 35% carbohydrate and 20% protein). Female C57B16/J mice were divided into two groups fed a high fat diet (HF) for 20 weeks supplemented or not with 1.5 g/kg/day of GOS (HF-GOS). The GOS supplementation did not change body weight nor fat pad mass, nor any of the blood parameters measured (glucose, insulin, leptin, triglycerides, and free fatty acids). However, mice which received the GOS supplemented diet showed an increased glucose utilization after a 1 g/kg load of glucose compared with the mice fed the high fat diet alone. Our results suggest a role for non-digestible GOS in the regulation of carbohydrate metabolism.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Adipose Tissue / drug effects
  • Adipose Tissue / metabolism
  • Animals
  • Blood Glucose / drug effects
  • Body Weight / drug effects
  • Dietary Fats / administration & dosage*
  • Dietary Fats / metabolism
  • Fatty Acids, Nonesterified / blood
  • Female
  • Glucose / analogs & derivatives
  • Glucose / metabolism*
  • Glucose Tolerance Test
  • Insulin / blood
  • Leptin / blood
  • Mice
  • Mice, Inbred C57BL
  • Oligosaccharides / chemistry
  • Oligosaccharides / pharmacology*
  • Triglycerides / blood


  • Blood Glucose
  • Dietary Fats
  • Fatty Acids, Nonesterified
  • Insulin
  • Leptin
  • Oligosaccharides
  • Triglycerides
  • Glucose